Uji Antidiare Ekstrak Metanol dan Ekstrak Akuades Daun Pepaya (Carica papaya L.) terhadap Tikus Galur Wistar yang Diinduksi Oleum ricini

Naesit Andre Darvid; Husnul Khuluq; Titi Pudji Rahayu

doi:10.57174/j.born.v3i3.107

Authors

Naesit Andre Darvid Fakultas Ilmu Kesehatan, Universitas Muhammadiyah Gombong
Husnul Khuluq Fakultas Ilmu Kesehatan, Universitas Muhammadiyah Gombong
Titi Pudji Rahayu Fakultas Ilmu Kesehatan, Universitas Muhammadiyah Gombong

DOI:

https://doi.org/10.57174/j.born.v3i3.107

Keywords:

antidiarrheal, papaya leaf, distilled water extract, methanol extract

Abstract

Diarrhea is a disease characterized by a change in the consistency of soft stool to liquid. The use of antidiarrheal drugs causes side effects such as stomach ache, nausea, or vomiting, so alternative treatments for diarrhea using natural ingredients are needed. The papaya plant (Carica papaya L.) is an herbal plant that can be used as an alternative treatment for diarrhea with fewer side effects. This study aimed to determine the antidiarrheal effect of methanol extract and aqueous extract of papaya leaves (Carica Papaya L.) on male white Wistar rats induced with Oleum ricini. This research is a laboratory experiment. The test animals were divided into 10 groups, namely negative control (CMC-Na 0.5%), positive control (Loperamide), methanol extract group, and distilled water extract with concentrations of 300, 450, and 600 mg/kgBW. Test animals were induced with Oleum ricini. The parameters observed include frequency, consistency, and weight of feces. The results of this research showed that methanol extract and distilled water at concentrations of 300 and 450 mg/kgBB were significantly able to reduce the frequency and increase the consistency of feces. Meanwhile, 300 mg/kgBB methanol extract and 600 mg/kgBB aquades extract were able to significantly reduce feces weight. The research conclusion shows that the best concentration of methanol extract and distilled water extract can have an antidiarrheal effect at concentrations of 300 mg/kgBW and 450 mg/kgBW to improve frequency and consistency. Methanol extract and distilled water extract 300 mg/kg and 600 mg/kgBW to reduce feces weight.

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References

Indijah SW, Fajri P. Modul Bahan Ajar Cetak Farmasi Farmakologi. Jakarta: Pusdik SDM Kesehatan. Kementerian Kesehatan Republik Indonesia; 2016.

Kemenkes RI. Laporan Riskesdas 2018 Kementerian Kesehatan Republik Indonesia [Internet]. Vol. 53, Laporan Nasional Riskesdas 2018. 2018. p. 154–65. Available from: http://www.yankes.kemkes.go.id/assets/downloads/PMK No. 57 Tahun 2013 tentang PTRM.pdf

Tjay TH, Rahardja K. Obat-Obat Penting Khasiat, Penggunaan dan Efek-Efek Sampingnya. Vol. 53, Pt Elex Media Komputindo. 2015. 522 p.

M.Sholekhudin. Buku obat sehari-hari. Jakarta: Elek Media Komputindo : Jakarta; 2014. 34 p.

Peristiowati Y, Puspitasari Y. Potensi Daun Pepaya. Sidoarjo : Indonesia Pustaka: Sidoarjo : Indonesia Pustaka; 2018.

Pratama M, Rosalina VS. Analisis Kadar Tanin Total EKstrak Etanol Bunga Cengkeh ( Syzygium aromaticum L .) Mengunakan Metode Spektrofotometri Uv-Vis. 2019;6(2):368–73.

Harningsih, Jumain, Ayatullah AG. Uji Efek Antidiare Ekstrak Etanol Daun Pepaya ( Caricapapaya L ) Terhadap Mencit (Mus musculus). Uji Efek Antidiare Ekstrak Etanol Daun Pepaya ( Caricapapaya L ) Terhadap Mencit (Mus musculus). 2016;

Manek MS. Uji Aktivitas Antidiare Ekstrak Etanol Daun Sirih (Piper betle L.) Pada Tikus Jantan Galur Wistar Yang DIinduksi Oleum Ricini. Skripsi. 2019;

Endarini LH. Modul Bahan Ajar Cetak Farmasi Farmakognosi dan Fitokimia. Jakarta: Pusdik SDM Kesehatan. Kementerian Kesehatan Republik Indonesia; 2016.

Khuluq H, Evi M. IAI CONFERENCE: Evaluation of antidiarrheal effect of combination of Salam Leaves (Syzygiumpolyanthum) and Jackfruit Leaves (Artocarpus heterophyllus Lam.) infusum in rats induced by castor oil. Pharm Educ [Internet]. 2021;21(2):148–151. Available from: https://pharmacyeducation.fip.org/pharmacyeducation/article/view/1424

Sukmawati IK, Yulinah Sukandar E, Fisheri Kurniati N. Aktivitas Antidiare Daun Harendong (Malestoma malabathricum L). J Syifa Sci Clin Res. 2020;2(1):39–48.

Hasan H, Ain Thomas N, Taupik M, Potabuga G. Efek Antelmintik Ekstrak Metanol Kulit Batang Nangka (Artocarpus heterophyllus) terhadap Cacing Ascaris lumbricoides. J Syifa Sci Clin Res. 2023;4(1):244–50.

Sa’adah H, Nurhasnawati H. Perbandingan Pelarut Etanol Dan Air Pembuatan Ektrak Umbi Bawang Tiwai (Eleutherine americana Merr) Menggunakan Metode Maserasi. J Ilm Manuntung. 2015;1(2):149.

Handayani PA, Ramadani NS, Kartika D. Pemungutan Tanin Propagul Mangrove (Rhizopora mucronata) dengan Pelarut Etanol dan Aquades Sebagai Zat Warna Alami Menggunakan Metode Microwave Assisted Extraction. J Kompetensi Tek. 2018;10(1):22–7.

Mariana 2018. Validasi Metode Penetapan Kuantitatif Metanol dalam Urin Menggunakan Gas Chromatography-Flame Ionization Detector. Indones J Chem Sci. 2018;7(3):277–84.

Luviana A, Putri A, Reynaldi R, Rahmawati SP, Azzahra RC, Sihombing RP, et al. Pengaruh Pelarut yang Digunakan terhadap Hasil Ekstraksi Kunyit ( Curcuma Longa L .). 2023;123–7.

Rambe R, Gultom ED, Ginting OSB, Diana S. Uji Efektivitas Antidiare Ekstrak Etanol Daun Mengkudu (Morinda citrifolia L.) Terhadap Mencit Jantan Dengan Metode Transit Intestinal. Forte J. 2021;1(1):01–11.