Identifikasi Senyawa Alkaloid dari Ekstrak Etanol Kulit Batang Strychnos ligustrina Menggunakan Liquid Chromatography-Mass Spectrometry (LC-MS)

Siti Wanda Nurwanti; Andriningrum Sarnoko; Atika Wulandari

doi:10.57174/j.born.v3i2.82

Authors

Siti Wanda Nurwanti Prodi Farmasi, Politeknik Hang Tuah Jakarta https://orcid.org/0009-0009-9225-887X
Andriningrum Sarnoko Prodi Farmasi, Politeknik Hang Tuah Jakarta
Atika Wulandari Prodi Farmasi, Politeknik Hang Tuah Jakarta https://orcid.org/0009-0009-9225-887X

DOI:

https://doi.org/10.57174/j.born.v3i2.82

Keywords:

3-iso-ajmalicine, bidara laut, brucine, Strychnos ligustrina

Abstract

Bidara Laut (S. ligustrina) stem bark is commonly used by the society of West Nusa Tenggara and Bali as an antimalaria treatment. The study aims to quantify the number of chemical components from the alkaloid family present in the ethanolic extract of S. ligustrina bark. With a 70% ethanol-based solvent, the bark of S. ligustrina was extracted using the maceration process. LC-MS was used to assess the number of alkaloids present in the bark of S. ligustrina. In a reverse phase column using acetonitrile and formic acid as the mobile phases, gradient elution was carried out for 16 minutes. Five alkaloid compounds were discovered as a consequence of the investigation, including the 3-iso-ajmalicine molecule, which had an m/z of 353.1847 and a retention time of 4.26 minutes, and the brucine compound, which had an m/z of 395.1953 and a retention time of 5.06 minutes. The other 3 molecules are confirmed with m/z of that molecules C₂₄H₂₈N₂O₄, C₂₂H₂₄N₂O₃, and C₁₁H₁₇NO₆ are 409.2107, 365.1848, and 260.1116, respectively, and their retention times are 4,90 minutes, 5,35 minutes, and 1,32 minutes. 3-iso-ajmalicine has just been confirmed to be present in S. ligustrina stem bark.

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References

Setiawan O, Narendra B H. Sistem perakaran bidara laut (Strychnos lucida R.Br.) untuk pengendalian tanah longsor (Strychnos lucida R.Br.) root system for landslide control. Jurnal Penelitian Kehutanan Wallacea.2012 ;12(8): 50-61.

Selfiana A. Identifikasi senyawa aktif antrakuinon fraksi etil asetat kayu songga (Strychnos ligustrina) sebagai anti malaria melalui uji aktivitas penghambat polimerisasi heme.[Yogyakarta]: Skripsi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Indonesia;2019.

Silva-leite K E S, Assreuy A M S, Mendonc L F, Damasceno L E A, Queiroz M G R, De Mourão P A S, Pires A F, Pereira M G. Polysaccharide rich fractions from barks of Ximenia americana inhibit peripheral inflammatory nociception in mice Antinociceptive effect of Ximenia americana p. Revista de Brasileira de Farmacognisia.2019;19(5):339-345.

Kurniawan E, Dyah J D S, Zulkifli L. Aktivitas antibakteri ekstrak metanol batang bidara laut (Strychnos ligustrina) terhadap bakteri patogen. Jurnal Biologi Tropis.2019;19(1): 61–69.

Setiawan O, Rostiwati T. Bidara Laut (Strychnos ligustrina Blume. syn. S. lucida R. Br): HHBK Potensial di NTB dan Bali. [Bogor]: Forda Press;2014.

Kurniadi K. The effect of boiled Sea Jujube (Strychnos ligustrina) on the reduction of blood glucose level in rats rendered to be diabetic by the induction with alloxan. Journal of Ners Community.2012; 3(1):98– 111.

Worachartcheewan A, Sarmento N, Pingaew R, Prachayasittikul S, Ruchirawat S, Prachayasittikul V. Antimicrobial, antioxidant and anticancer activities of Strychnos lucida. African Journal of Traditional, Complementary and Alternative Medicines.2015;12: 122-127.

Rahayu A A D, Prihantini A I, Krisnawati, Nugraheni Y M M A. Chemical components of different part of strychnos ligustrina, a medicinal plants from Indonesia. IOP Conf. Series: Earth and Environmental Science;2022.

Ekasari W, Aty W, Suhintam P. Uji anti malaria senyawa hasil isolasi fraksi positif alkaloid daun C. Siamea pada biakan in vitro P.Falciparum. [Surabaya]: Penelitian Dosen Muda/BBI, Lembaga Penelitian Universitas Airlangga; 2004.

Uzor P F. Alkaloids from plants with antimalarial activity: A review of recent studies. Hindawi Evidence-Based Complementary and Alternative Medicine;2020.

Kumar P R, Dinesh S R, Rini R. LCMS- a Review and a Recent Update. World Journal of Pharmacy and Pharmaceutical Sciences.2018;5(5): 377-391.

Heyne, K. Tumbuhan Berguna Indonesia III : Badan Penelitian dan Pengembangan Kehutanan. Jakarta: Departemen Kehutanan;1987.

Ginting, M K. Validasi Metode LC-MS/MS Untuk Penentuan Senyawa Asam Trans, Trans-Mukonat, Asam Hippurat, Asam 2-Metil Hippurat, Asam 3-Metil Hippurat, Asam 4-Metil Hippurat Dalam Urin Sebagai Biomarker Paparan Benzena, Toluena, dan Xilena. Depok: Skripsi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Indonesia;2012.

Sumiati E. Uji aktivitas antibakteri ekstrak kloroform dan ekstrak etanol biji bidara laut (Strychnos ligustrina) Terhadap Staphylococcus aureus ATCC 25923 dan Salmonella thypi. Jurnal Ilmia Biologi Biogenesis.2014;2(1):1-10.

Fadhly E, Kusrini D, Fachriyah E. Isolasi, identifikasi, senyawa alkaloid dari daun Rivina humilis L. serta uji sitotoksik menggunakan metode BLST (Brine Shrimp Lethality Test). Jurnal Kimia Sains dan Aplikasi.2015;18(2);67-72

Taufik M, Djuwarno E N, Mustafa M A, Sahumena M H. Identifikasi dan studi pola fragmentasi jamu terkonfirmasi fenilbutazon menggunakan liquid chromatography mass spectroscopy (Lcms). SCIENTIA Jurnal Farmasi dan Kesehatan.2020;10(2).

Untoro M, Fachriyah E, Kusrini D. Isolasi dan identifikasi senyawa golongan alkaloid dari rimpang lengkuas merah (Alpinia purpurata). Jurnal Kimia Sains dan Aplikasi. 2016;19(2):58-62

Nurhasnawati H, Sukarmi, Handayani F. Perbandingan metode ekstraksi maserasi dan sokletasi terhadap aktivitas antioksidan ekstrak etanol daun jambu bol (Syzygium malaccense L.). Jurnal Ilmiah Manuntung.2017;3(1):91-95.

Najiyyah Siti Ulfatun, dkk. Gelas kayu bidara laut (Geyu Bilut) sebagai antimalaria. NTB: Pendidikan Dokter, Fakultas Kedokteran Universitas Mataram;2014.

Ratnasari J, Siregar A H, Rizkita R E. Pengaruh pemberian elisitor ekstrak khamir saccharomyces cerevisiae hansen terhadap kandungan ajmalisin dalam kultur agregat catharanthus roseus (L.) G. Don. Berita Biologi.2001;5(4).

Shi, L., Li, Y., Fei, L., and Lv, S. Herbal textual research on Strychnos nuxvomica. Res. Pract. Chin. Medicines.2017:31(06):6–10.

Lu L, Huang R, Wu Y, Jin J M, Chen H Z, Zhang L Z, Luan X. Brucine: A review of pythochemistry, pharmacology and toxicology. Frontiers in Pharmacology;2020.

National Center for Biotechnology Information (2023). PubChem Compound Summary for CID 85540149. Retrieved May 4, 2023 from https://pubchem.ncbi.nlm.nih.gov/compound/85540149.

National Center for Biotechnology Information (2023). PubChem Compound Summary for CID 94643115, CID 94643115. Retrieved May 4, 2023 from https://pubchem.ncbi.nlm.nih.gov/compound/94643115.

National Center for Biotechnology Information (2023). PubChem Compound Summary for CID 16754231, 1-O-Propargyl-N-acetyl-beta-D-glucosamine. Retrieved May 4, 2023 from https://pubchem.ncbi.nlm.nih.gov/compound/1-O-Propargyl-N-acetyl-beta-D-glucosamine.